Review



dio2 overexpression plasmids  (Genecopoeia)


Bioz Verified Symbol Genecopoeia is a verified supplier
Bioz Manufacturer Symbol Genecopoeia manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Genecopoeia dio2 overexpression plasmids
    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of <t>DIO2</t> , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups
    Dio2 Overexpression Plasmids, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dio2+overexpression+plasmids/SOX9+Rabbit+mAb/pmc12964726-126-4-7
    Average 94 stars, based on 4 article reviews
    dio2 overexpression plasmids - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis"

    Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-026-07866-z

    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of DIO2 , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups
    Figure Legend Snippet: Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of DIO2 , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups

    Techniques Used: Functional Assay, Expressing, Control

    DIO2 ⁺ Str4 stromal cells exhibit SASP-like activity and drive decidualization. ( A , B ) Representative immunohistochemistry (IHC) images of DIO2, CXCL12, IGFBP3, and MMP14 in mid-secretory eutopic endometrial tissues from controls and adenomyosis (AM) patients ( n = 12). Scale bars, 50 μm. ( C ) ELISA quantification of decidualization markers IGFBP1 and PRL in control and AM tissues ( n = 12). ( D ) Senescence-associated β-galactosidase (SA-β-gal) staining in control HESCs undergoing in vitro decidualization. ( E ) RT-qPCR analysis of SASP-related cytokines CXCL14, TIMP3, and IL15 in decidualized control HESCs ( n = 3). ( F ) ELISA measurement of secreted SASP cytokines in culture supernatant ( n = 3). ( G – K ) Effects of DIO2 knockdown on β-gal activity ( G ), SASP cytokine mRNA expression ( H ), and secreted protein levels ( I – K ) in control HESCs ( n = 3). ( L , M ) F-actin filaments staining in control HESCs following DIO2 knockdown during in vitro decidualization, visualized with Alexa Fluor 555–conjugated phalloidin. Scale bar = 50 μm, n = 3. Data are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Figure Legend Snippet: DIO2 ⁺ Str4 stromal cells exhibit SASP-like activity and drive decidualization. ( A , B ) Representative immunohistochemistry (IHC) images of DIO2, CXCL12, IGFBP3, and MMP14 in mid-secretory eutopic endometrial tissues from controls and adenomyosis (AM) patients ( n = 12). Scale bars, 50 μm. ( C ) ELISA quantification of decidualization markers IGFBP1 and PRL in control and AM tissues ( n = 12). ( D ) Senescence-associated β-galactosidase (SA-β-gal) staining in control HESCs undergoing in vitro decidualization. ( E ) RT-qPCR analysis of SASP-related cytokines CXCL14, TIMP3, and IL15 in decidualized control HESCs ( n = 3). ( F ) ELISA measurement of secreted SASP cytokines in culture supernatant ( n = 3). ( G – K ) Effects of DIO2 knockdown on β-gal activity ( G ), SASP cytokine mRNA expression ( H ), and secreted protein levels ( I – K ) in control HESCs ( n = 3). ( L , M ) F-actin filaments staining in control HESCs following DIO2 knockdown during in vitro decidualization, visualized with Alexa Fluor 555–conjugated phalloidin. Scale bar = 50 μm, n = 3. Data are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Techniques Used: Activity Assay, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control, Staining, In Vitro, Quantitative RT-PCR, Knockdown, Expressing

    Related Articles

    Control:

    Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis
    Article Snippet: .. Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA). .. Gene-specific siRNAs targeting DIO2, LGR5, or SOX9, or a scrambled control siRNA (15 nM; GenePharma, China), were transfected using Lipofectamine RNAiMAX (Invitrogen, USA) according to the manufacturer’s instructions. siRNA sequences are listed in Supplementary Table .

    Over Expression:

    Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis
    Article Snippet: .. Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA). .. Gene-specific siRNAs targeting DIO2, LGR5, or SOX9, or a scrambled control siRNA (15 nM; GenePharma, China), were transfected using Lipofectamine RNAiMAX (Invitrogen, USA) according to the manufacturer’s instructions. siRNA sequences are listed in Supplementary Table .



    Similar Products

    dio2 overexpression plasmids  (Genecopoeia)
    94
    Genecopoeia dio2 overexpression plasmids
    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of <t>DIO2</t> , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups
    Dio2 Overexpression Plasmids, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dio2+overexpression+plasmids/SOX9+Rabbit+mAb/pmc12964726-126-4-7
    Average 94 stars, based on 1 article reviews
    dio2 overexpression plasmids - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    dio2 overexpression plasmid  (Obio Technology Corp Ltd)
    86
    Obio Technology Corp Ltd dio2 overexpression plasmid
    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of <t>DIO2</t> , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups
    Dio2 Overexpression Plasmid, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dio2+overexpression+plasmids/dio2+overexpression+plasmid/pm41331484-149-10-18
    Average 86 stars, based on 1 article reviews
    dio2 overexpression plasmid - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    the dio2-overexpression plasmid  (Genecopoeia)
    90
    Genecopoeia the dio2-overexpression plasmid
    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of <t>DIO2</t> , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups
    The Dio2 Overexpression Plasmid, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dio2+overexpression+plasmids/dio2/pm34166926-93-1-6
    Average 90 stars, based on 1 article reviews
    the dio2-overexpression plasmid - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of DIO2 , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups

    Journal: Journal of Translational Medicine

    Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis

    doi: 10.1186/s12967-026-07866-z

    Figure Lengend Snippet: Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of DIO2 , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups

    Article Snippet: Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA).

    Techniques: Functional Assay, Expressing, Control

    DIO2 ⁺ Str4 stromal cells exhibit SASP-like activity and drive decidualization. ( A , B ) Representative immunohistochemistry (IHC) images of DIO2, CXCL12, IGFBP3, and MMP14 in mid-secretory eutopic endometrial tissues from controls and adenomyosis (AM) patients ( n = 12). Scale bars, 50 μm. ( C ) ELISA quantification of decidualization markers IGFBP1 and PRL in control and AM tissues ( n = 12). ( D ) Senescence-associated β-galactosidase (SA-β-gal) staining in control HESCs undergoing in vitro decidualization. ( E ) RT-qPCR analysis of SASP-related cytokines CXCL14, TIMP3, and IL15 in decidualized control HESCs ( n = 3). ( F ) ELISA measurement of secreted SASP cytokines in culture supernatant ( n = 3). ( G – K ) Effects of DIO2 knockdown on β-gal activity ( G ), SASP cytokine mRNA expression ( H ), and secreted protein levels ( I – K ) in control HESCs ( n = 3). ( L , M ) F-actin filaments staining in control HESCs following DIO2 knockdown during in vitro decidualization, visualized with Alexa Fluor 555–conjugated phalloidin. Scale bar = 50 μm, n = 3. Data are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Journal of Translational Medicine

    Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis

    doi: 10.1186/s12967-026-07866-z

    Figure Lengend Snippet: DIO2 ⁺ Str4 stromal cells exhibit SASP-like activity and drive decidualization. ( A , B ) Representative immunohistochemistry (IHC) images of DIO2, CXCL12, IGFBP3, and MMP14 in mid-secretory eutopic endometrial tissues from controls and adenomyosis (AM) patients ( n = 12). Scale bars, 50 μm. ( C ) ELISA quantification of decidualization markers IGFBP1 and PRL in control and AM tissues ( n = 12). ( D ) Senescence-associated β-galactosidase (SA-β-gal) staining in control HESCs undergoing in vitro decidualization. ( E ) RT-qPCR analysis of SASP-related cytokines CXCL14, TIMP3, and IL15 in decidualized control HESCs ( n = 3). ( F ) ELISA measurement of secreted SASP cytokines in culture supernatant ( n = 3). ( G – K ) Effects of DIO2 knockdown on β-gal activity ( G ), SASP cytokine mRNA expression ( H ), and secreted protein levels ( I – K ) in control HESCs ( n = 3). ( L , M ) F-actin filaments staining in control HESCs following DIO2 knockdown during in vitro decidualization, visualized with Alexa Fluor 555–conjugated phalloidin. Scale bar = 50 μm, n = 3. Data are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA).

    Techniques: Activity Assay, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control, Staining, In Vitro, Quantitative RT-PCR, Knockdown, Expressing